Ischemia/reperfusion injury of porcine limbs after extracorporeal perfusion

Müller, Sabine; Constantinescu, Mihai A; Kiermeir, David M; Gajanayake, Thusitha; Bongoni, Anjan K; Vollbach, Felix H; Meoli, Martino; Plock, Jan; Jenni, Hansjörg; Banic, Andrej; Rieben, Robert; Vögelin, Esther

doi:10.1016/j.jss.2012.05.088

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Müller, S., Constantinescu, M. A., Kiermeir, D. M., Gajanayake, T., Bongoni, A. K., Vollbach, F. H., Meoli, M., Plock, J., Jenni, H., Banic, A., Rieben, R., & Vögelin, E. (2013). Ischemia/reperfusion injury of porcine limbs after extracorporeal perfusion. Journal of Surgical Research, 181(1), 170–182. https://doi.org/10.1016/j.jss.2012.05.088

Abstract

BACKGROUND: Revascularization of amputated extremities after prolonged ischemia is complicated by reperfusion injury. We assessed ischemia/reperfusion (I/R) injury of porcine extremities after prolonged preservation using extracorporeal circulation (ECC). METHODS: Forelimbs of 32 pigs were divided into four groups based on ischemia times: group I: 6 h, group II: 12 h, group III: 0 h plus replantation, and group IV: 6 h plus replantation. Limbs were perfused with autologous blood using ECC for 12 h except group II with only 5 h perfusi