Publication: Knowledge-based design of a biosensor to quantify localized ERK activation in living cells
Knowledge-based design of a biosensor to quantify localized ERK activation in living cells
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Kummer, L., Hsu, C.-W., Dagliyan, O., Macnevin, C., Kaufholz, M., Zimmermann, B., Dokholyan, N. V., Hahn, K. M., & Plückthun, A. (2013). Knowledge-based design of a biosensor to quantify localized ERK activation in living cells. Chemistry & Biology, 20(6), 847–856. https://doi.org/10.1016/j.chembiol.2013.04.016
Abstract
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Abstract
Investigation of protein activation in living cells is fundamental to understanding how proteins are influenced by the full complement of upstream regulators they experience. Here, we describe the generation of a biosensor based on the DARPin binding scaffold suited for intracellular applications. Combining library selection and knowledge-based design, we created an ERK activity biosensor by derivatizing a DARPin specific for phosphorylated ERK with a solvatochromatic merocyanine dye, whose fluorescence increases upon pERK binding. Th
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Creators (Authors)
- Kummer, Lutz
- Hsu, Chia-Wen
- Dagliyan, Onur
- Macnevin, Christopher
- Kaufholz, Melanie
- Zimmermann, Bastian
- Dokholyan, Nikolay V
- Hahn, Klaus M
- Plückthun, Andreas
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Citations
Kummer, L., Hsu, C.-W., Dagliyan, O., Macnevin, C., Kaufholz, M., Zimmermann, B., Dokholyan, N. V., Hahn, K. M., & Plückthun, A. (2013). Knowledge-based design of a biosensor to quantify localized ERK activation in living cells. Chemistry & Biology, 20(6), 847–856. https://doi.org/10.1016/j.chembiol.2013.04.016
