Publication: FRAP analysis of LET-23::GFP in the vulval epithelial cells of living caenorhabditis elegans larvae
FRAP analysis of LET-23::GFP in the vulval epithelial cells of living caenorhabditis elegans larvae
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Walser, M., Hajnal, A., & Escobar-Restrepo, J. M. (2015). FRAP analysis of LET-23::GFP in the vulval epithelial cells of living caenorhabditis elegans larvae. Bio-Protocol, 5(11), e1489. http://www.bio-protocol.org/e1489
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The Caenorhabditis elegans (C. elegans) vulva is a well-established system to study organ development as the molecular mechanisms that govern its formation are conserved in animals. Of special interest is the EGFR/RAS/MAPK signaling pathway that is required for fate acquisition and morphogenesis of the vulva. let-23 encodes the sole homologue of the epidermal growth factor receptor (EGFR), is expressed at the plasma membrane of the vulval precursor cells (VPCs) and is activated by LIN-3 EGF at the end of the L3 larval stage to initiat
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Walser, M., Hajnal, A., & Escobar-Restrepo, J. M. (2015). FRAP analysis of LET-23::GFP in the vulval epithelial cells of living caenorhabditis elegans larvae. Bio-Protocol, 5(11), e1489. http://www.bio-protocol.org/e1489
