Comparison of Disk Diffusion, E-Test, and Broth Microdilution Methods for Testing In Vitro Activity of Cefiderocol in Acinetobacter baumannii

Kolesnik-Goldmann, Natalia; Seth-Smith, Helena M B; Haldimann, Klara; Imkamp, Frank; Roloff, Tim; Zbinden, Reinhard; Hobbie, Sven N; Egli, Adrian; Mancini, Stefano

doi:10.3390/antibiotics12071212

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Kolesnik-Goldmann, N., Seth-Smith, H. M. B., Haldimann, K., Imkamp, F., Roloff, T., Zbinden, R., Hobbie, S. N., Egli, A., & Mancini, S. (2023). Comparison of Disk Diffusion, E-Test, and Broth Microdilution Methods for Testing In Vitro Activity of Cefiderocol in Acinetobacter baumannii. Antibiotics, 12(7), 1212. https://doi.org/10.3390/antibiotics12071212

Abstract

The reference method for cefiderocol antimicrobial susceptibility testing is broth microdilution (BMD) with iron-depleted-Mueller-Hinton (ID-MH) medium, whereas breakpoints recommended for disk diffusion (DD) are based on MH-agar plates. We aimed to compare the performance of the commercial BMD tests ComASP (Liofilchem) and UMIC (Bruker), and DD and E-test using MH- and ID-MH-agar plates with the reference BMD method using 100 carbapenem-resistant-A. baumannii isolates. Standard BMD was performed according to the EUCAST guidelines; DD