Publication: A platinum-based covalent viability reagent for single-cell mass cytometry
A platinum-based covalent viability reagent for single-cell mass cytometry
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Fienberg, H. G., Simonds, E. F., Fantl, W. J., Nolan, G. P., & Bodenmiller, B. (2012). A platinum-based covalent viability reagent for single-cell mass cytometry. Cytometry. Part A, 81A(6), 467–475. https://doi.org/10.1002/cyto.a.22067
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In fluorescence-based flow cytometry, cellular viability is determined with membrane-impermeable fluorescent reagents that specifically enter and label plasma membrane-compromised nonviable cells. A recent technological advance in flow cytometry uses antibodies conjugated to elemental metal isotopes, rather than to fluorophores, to allow signal detection by atomic mass spectrometry. Unhampered by the limitations of overlapping emission fluorescence, mass cytometry increases the number of parameters that can be measured in single cells
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- Fienberg, Harris G
- Simonds, Erin F
- Fantl, Wendy J
- Nolan, Garry P
- Bodenmiller, Bernd
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Fienberg, H. G., Simonds, E. F., Fantl, W. J., Nolan, G. P., & Bodenmiller, B. (2012). A platinum-based covalent viability reagent for single-cell mass cytometry. Cytometry. Part A, 81A(6), 467–475. https://doi.org/10.1002/cyto.a.22067