Developmental validation of Oxford Nanopore Technology MinION sequence data and the NGSpeciesID bioinformatic pipeline for forensic genetic species identification

Vasiljevic, Nina; Lim, Marisa; Humble, Emily; Seah, Adeline; Kratzer, Adelgunde; Morf, Nadja V; Prost, Stefan; Ogden, Rob

doi:10.1016/j.fsigen.2021.102493

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Vasiljevic, N., Lim, M., Humble, E., Seah, A., Kratzer, A., Morf, N. V., Prost, S., & Ogden, R. (2021). Developmental validation of Oxford Nanopore Technology MinION sequence data and the NGSpeciesID bioinformatic pipeline for forensic genetic species identification. Forensic Science International. Genetics, 53, 102493. https://doi.org/10.1016/j.fsigen.2021.102493

Abstract

Species identification of non-human biological evidence through DNA nucleotide sequencing is routinely used for forensic genetic analysis to support law enforcement. The gold standard for forensic genetics is conventional Sanger sequencing; however, this is gradually being replaced by high-throughput sequencing (HTS) approaches which can generate millions of individual reads in a single experiment. HTS sequencing, which now dominates molecular biology research, has already been demonstrated for use in a number of forensic genetic anal