Publication: Bacterial diversity and community composition in the chemocline of the meromictic alpine Lake Cadagno as revealed by 16S rDNA analysis
Bacterial diversity and community composition in the chemocline of the meromictic alpine Lake Cadagno as revealed by 16S rDNA analysis
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Bosshard, P. P., Santini, Y., Grüter, D., Stettler, R., & Bachofen, R. (2000). Bacterial diversity and community composition in the chemocline of the meromictic alpine Lake Cadagno as revealed by 16S rDNA analysis. FEMS Microbiology Ecology, 31, 173–182. https://doi.org/10.1111/j.1574-6941.2000.tb00682.x
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Using different techniques of molecular biology we investigated the bacterial diversity of the chemocline of the meromictic Lake Cadagno. Cloning of a total community 16S rDNA PCR product and subsequent screening with a combination of amplified ribosomal DNA restriction analysis and temporal temperature gradient gel electrophoresis (TTGE) analysis revealed that 30 of 47 randomly selected clones were unique. Partial sequencing and comparative analysis indicated a high bacterial diversity dominated by the γ-Proteobacteria (33.3%). Most
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Bosshard, P. P., Santini, Y., Grüter, D., Stettler, R., & Bachofen, R. (2000). Bacterial diversity and community composition in the chemocline of the meromictic alpine Lake Cadagno as revealed by 16S rDNA analysis. FEMS Microbiology Ecology, 31, 173–182. https://doi.org/10.1111/j.1574-6941.2000.tb00682.x