Publication: Quantifying Variation in Bacterial Reproductive Fitness: a High-Throughput Method
Quantifying Variation in Bacterial Reproductive Fitness: a High-Throughput Method
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Frey, P. M., Baer, J., Bergada-Pijuan, J., Lawless, C., Bühler, P. K., Kouyos, R. D., Lemon, K. P., Zinkernagel, A. S., & Brugger, S. D. (2021). Quantifying Variation in Bacterial Reproductive Fitness: a High-Throughput Method. MSystems, 6, e01323-20. https://doi.org/10.1128/mSystems.01323-20
Abstract
Abstract
Abstract
To evaluate changes in reproductive fitness of bacteria, e.g., after acquisition of antimicrobial resistance, a low-cost high-throughput method to analyze bacterial growth on agar is desirable for broad usability. In our bacterial quantitative fitness analysis (BaQFA), arrayed cultures are spotted on agar and photographed sequentially while growing. These time-lapse images are analyzed using a purpose-built open-source software to derive normalized image intensity (NI) values for each culture spot. Subsequently, a Gompertz growth mode
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- Frey, Pascal M
- Baer, Julian
- Bergada-Pijuan, Judith
- Lawless, Conor
- Bühler, Philipp K
- Kouyos, Roger D
- Lemon, Katherine P
- Zinkernagel, Annelies S
- Brugger, Silvio D
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Frey, P. M., Baer, J., Bergada-Pijuan, J., Lawless, C., Bühler, P. K., Kouyos, R. D., Lemon, K. P., Zinkernagel, A. S., & Brugger, S. D. (2021). Quantifying Variation in Bacterial Reproductive Fitness: a High-Throughput Method. MSystems, 6, e01323-20. https://doi.org/10.1128/mSystems.01323-20
