Publication: GroEL/S Overexpression Helps to Purge Deleterious Mutations and Reduce Genetic Diversity during Adaptive Protein Evolution
GroEL/S Overexpression Helps to Purge Deleterious Mutations and Reduce Genetic Diversity during Adaptive Protein Evolution
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Iyengar, B. R., & Wagner, A. (2022). GroEL/S Overexpression Helps to Purge Deleterious Mutations and Reduce Genetic Diversity during Adaptive Protein Evolution. Molecular Biology and Evolution, 39(6), msac047. https://doi.org/10.1093/molbev/msac047
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Chaperones are proteins that help other proteins fold. They also affect the adaptive evolution of their client proteins by buffering the effect of deleterious mutations and increasing the genetic diversity of evolving proteins. We study how the bacterial chaperone GroE (GroEL+GroES) affects the evolution of green fluorescent protein (GFP). To this end, we subjected GFP to multiple rounds of mutation and selection for its color phenotype in four replicate Escherichia coli populations, and studied its evolutionary dynamics through high-
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- Iyengar, Bharat Ravi
- Wagner, Andreas
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Iyengar, B. R., & Wagner, A. (2022). GroEL/S Overexpression Helps to Purge Deleterious Mutations and Reduce Genetic Diversity during Adaptive Protein Evolution. Molecular Biology and Evolution, 39(6), msac047. https://doi.org/10.1093/molbev/msac047
