Publication: Imaging Flow Cytometry of Legionella-Containing Vacuoles in Intact and Homogenized Wild-Type and Mutant Dictyostelium
Imaging Flow Cytometry of Legionella-Containing Vacuoles in Intact and Homogenized Wild-Type and Mutant Dictyostelium
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Welin, A., Hüsler, D., & Hilbi, H. (2023). Imaging Flow Cytometry of Legionella-Containing Vacuoles in Intact and Homogenized Wild-Type and Mutant Dictyostelium. In N. Barteneva & I. Vorobjev (Eds.), Spectral and Imaging Cytometry : Methods and Protocols (No. 2635; Vol. 2635, pp. 63–85). Springer. https://doi.org/10.1007/978-1-0716-3020-4_4
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The causative agent of a severe pneumonia termed "Legionnaires' disease", Legionella pneumophila, replicates within protozoan and mammalian phagocytes in a specialized intracellular compartment called the Legionella-containing vacuole (LCV). This compartment does not fuse with bactericidal lysosomes but communicates extensively with several cellular vesicle trafficking pathways and eventually associates tightly with the endoplasmic reticulum. In order to comprehend in detail the complex process of LCV formation, the identification and
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- Welin, Amanda
- Hüsler, Dario
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Welin, A., Hüsler, D., & Hilbi, H. (2023). Imaging Flow Cytometry of Legionella-Containing Vacuoles in Intact and Homogenized Wild-Type and Mutant Dictyostelium. In N. Barteneva & I. Vorobjev (Eds.), Spectral and Imaging Cytometry : Methods and Protocols (No. 2635; Vol. 2635, pp. 63–85). Springer. https://doi.org/10.1007/978-1-0716-3020-4_4
