RAD51 Inhibition Induces R-Loop Formation in Early G1 Phase of the Cell Cycle

Nascakova, Zuzana; Boleslavska, Barbora; Urban, Vaclav; Oravetzova, Anna; Vlachova, Edita; Janscak, Pavel; Dobrovolna, Jana

doi:10.3390/ijms22073740

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Nascakova, Z., Boleslavska, B., Urban, V., Oravetzova, A., Vlachova, E., Janscak, P., & Dobrovolna, J. (2021). RAD51 Inhibition Induces R-Loop Formation in Early G1 Phase of the Cell Cycle. International Journal of Molecular Sciences, 22(7), 3740. https://doi.org/10.3390/ijms22073740

Abstract

R-loops are three-stranded structures generated by annealing of nascent transcripts to the template DNA strand, leaving the non-template DNA strand exposed as a single-stranded loop. Although R-loops play important roles in physiological processes such as regulation of gene expression, mitochondrial DNA replication, or immunoglobulin class switch recombination, dysregulation of the R-loop metabolism poses a threat to the stability of the genome. A previous study in yeast has shown that the homologous recombination machinery contribute