Publication: Generation of genome-modified Drosophila cell lines using SwAP
Generation of genome-modified Drosophila cell lines using SwAP
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Franz, A., Brunner, E., & Basler, K. (2017). Generation of genome-modified Drosophila cell lines using SwAP. Fly, 11(4), 303–311. https://doi.org/10.1080/19336934.2017.1372068
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The ease of generating genetically modified animals and cell lines has been markedly increased by the recent development of the versatile CRISPR/Cas9 tool. However, while the isolation of isogenic cell populations is usually straightforward for mammalian cell lines, the generation of clonal Drosophila cell lines has remained a longstanding challenge, hampered by the difficulty of getting Drosophila cells to grow at low densities. Here, we describe a highly efficient workflow to generate clonal Cas9-engineered Drosophila cell lines usi
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- Franz, Alexandra
- Brunner, Erich
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Franz, A., Brunner, E., & Basler, K. (2017). Generation of genome-modified Drosophila cell lines using SwAP. Fly, 11(4), 303–311. https://doi.org/10.1080/19336934.2017.1372068
