Single-cell proteomics defines the cellular heterogeneity of localized prostate cancer

de Vargas Roditi, Laura; Jacobs, Andrea; Rueschoff, Jan H; Bankhead, Pete; Chevrier, Stéphane; Jackson, Hartland Warren; Hermanns, Thomas; Fankhauser, Christian Daniel; Poyet, Cédric; Chun, Felix; Rupp, Niels J; Tschaebunin, Alexandra; Bodenmiller, Bernd; Wild, Peter Johannes

doi:10.1016/j.xcrm.2022.100604

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de Vargas Roditi, L., Jacobs, A., Rueschoff, J. H., Bankhead, P., Chevrier, S., Jackson, H. W., Hermanns, T., Fankhauser, C. D., Poyet, C., Chun, F., Rupp, N. J., Tschaebunin, A., Bodenmiller, B., & Wild, P. J. (2022). Single-cell proteomics defines the cellular heterogeneity of localized prostate cancer. Cell Reports Medicine, 3(4), 100604. https://doi.org/10.1016/j.xcrm.2022.100604

Abstract

Localized prostate cancer exhibits multiple genomic alterations and heterogeneity at the proteomic level. Single-cell technologies capture important cell-to-cell variability responsible for heterogeneity in biomarker expression that may be overlooked when molecular alterations are based on bulk tissue samples. This study aims to identify prognostic biomarkers and describe the heterogeneity of prostate cancer and the associated microenvironment by simultaneously quantifying 36 proteins using single-cell mass cytometry analysis of over