Publication: Photocontrolling Protein–Peptide Interactions: From Minimal Perturbation to Complete Unbinding
Photocontrolling Protein–Peptide Interactions: From Minimal Perturbation to Complete Unbinding
Date
Date
Date
Citations
Jankovic, B., Gulzar, A., Zanobini, C., Bozovic, O., Wolf, S., Stock, G., & Hamm, P. (2019). Photocontrolling Protein–Peptide Interactions: From Minimal Perturbation to Complete Unbinding. Journal of the American Chemical Society, 141, 10702–10710. https://doi.org/10.1021/jacs.9b03222
Abstract
Abstract
Abstract
An azobenzene-derived photoswitch has been covalently cross-linked to two sites of the S-peptide in the RNase S complex in a manner that the alpha-helical content of the S-peptide reduces upon cis-to-trans isomerization of the photoswitch. Three complementary experimental techniques have been employed, isothermal titration calorimetry, circular dichroism spectroscopy and intrinsic tyrosine fluorescence quenching, to determine the binding affinity of the S-peptide to the S-protein in the two states of the photoswitch. Five mutants with
Additional indexing
Creators (Authors)
- Jankovic, Brankica
- Gulzar, Adnan
- Zanobini, Claudio
- Bozovic, Olga
- Wolf, Steffen
- Stock, Gerhard
Journal/Series Title
Journal/Series Title
Journal/Series Title
Volume
Volume
Volume
Number
Number
Number
Page range/Item number
Page range/Item number
Page range/Item number
Page end
Page end
Page end
Item Type
Item Type
Item Type
In collections
Dewey Decimal Classifikation
Dewey Decimal Classifikation
Dewey Decimal Classifikation
Language
Language
Language
Publication date
Publication date
Publication date
Date available
Date available
Date available
ISSN or e-ISSN
ISSN or e-ISSN
ISSN or e-ISSN
OA Status
OA Status
OA Status
Publisher DOI
Citations
Jankovic, B., Gulzar, A., Zanobini, C., Bozovic, O., Wolf, S., Stock, G., & Hamm, P. (2019). Photocontrolling Protein–Peptide Interactions: From Minimal Perturbation to Complete Unbinding. Journal of the American Chemical Society, 141, 10702–10710. https://doi.org/10.1021/jacs.9b03222
