Publication: Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord
Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord
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Haenraets, K., Albisetti, G. W., Foster, E., & Wildner, H. (2018). Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord. Journal of Visualized Experiments, 135, e57382. https://doi.org/10.3791/57382
Abstract
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Abstract
Selective manipulation of spinal neuronal subpopulations has mainly been achieved by two different methods: 1) Intersectional genetics, whereby double or triple transgenic mice are generated in order to achieve selective expression of a reporter or effector gene (e.g., from the Rosa26 locus) in the desired spinal population. 2) Intraspinal injection of Cre-dependent recombinant adeno-associated virus (rAAV); here Cre-dependent AAV vectors coding for the reporter or effector gene of choice are injected into the spinal cord of mice expr
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- Haenraets, Karen
- Albisetti, Gioele W
- Foster, Edmund
- Wildner, Hendrik
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Haenraets, K., Albisetti, G. W., Foster, E., & Wildner, H. (2018). Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord. Journal of Visualized Experiments, 135, e57382. https://doi.org/10.3791/57382
