Publication: Quantitative TaqMan(R) real-time PCR assays for gene expression normalisation in feline tissues
Quantitative TaqMan(R) real-time PCR assays for gene expression normalisation in feline tissues
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Kessler, Y., Helfer-Hungerbuehler, A. K., Cattori, V., Meli, M. L., Zellweger, B., Ossent, P., Riond, B., Reusch, C. E., Lutz, H., & Hofmann-Lehmann, R. (2009). Quantitative TaqMan(R) real-time PCR assays for gene expression normalisation in feline tissues. BMC Molecular Biology, 10(1), 106. https://doi.org/10.1186/1471-2199-10-106
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ABSTRACT: BACKGROUND: Gene expression analysis is an important tool in contemporary research, with real-time PCR as the method of choice for quantifying transcription levels. Co-analysis of suitable reference genes is crucial for accurate expression normalisation. Reference gene expression may vary, e.g., among species or tissues; thus, candidate genes must be tested prior to use in expression studies. The domestic cat is an important study subject in both medical research and veterinary medicine. The aim of the present study was to d
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Kessler, Y., Helfer-Hungerbuehler, A. K., Cattori, V., Meli, M. L., Zellweger, B., Ossent, P., Riond, B., Reusch, C. E., Lutz, H., & Hofmann-Lehmann, R. (2009). Quantitative TaqMan(R) real-time PCR assays for gene expression normalisation in feline tissues. BMC Molecular Biology, 10(1), 106. https://doi.org/10.1186/1471-2199-10-106