Publication: Assessment of six different collagenase-based methods to isolate feline pancreatic islets
Assessment of six different collagenase-based methods to isolate feline pancreatic islets
Date
Date
Date
Citations
Zini, E., Franchini, M., Guscetti, F., Osto, M., Kaufmann, K., Ackermann, M., Lutz, T. A., & Reusch, C. E. (2009). Assessment of six different collagenase-based methods to isolate feline pancreatic islets. Research in Veterinary Science, 87(3), 367–372. https://doi.org/10.1016/j.rvsc.2009.03.017
Abstract
Abstract
Abstract
Isolation of pancreatic islets is necessary to study the molecular mechanisms underlying beta-cell demise in diabetic cats. Six collagenase-based methods of isolation were compared in 10 cat pancreata, including single and double course of collagenase, followed or not by Ficoll centrifugation or accutase, and collagenase plus accutase. Morphometric analysis was performed to measure the relative area of islet and exocrine tissue. Islet specific mRNA transcripts were quantified in isolates by real-time PCR. The single and double course
Metrics
Downloads
Views
Additional indexing
Creators (Authors)
- Zini, Eric
- Franchini, M
- Guscetti, Franco
- Osto, Melania
- Kaufmann, K
- Ackermann, M
- Reusch, Claudia E
Volume
Volume
Volume
Number
Number
Number
Page range/Item number
Page range/Item number
Page range/Item number
Page end
Page end
Page end
Item Type
Item Type
Item Type
Language
Language
Language
Publication date
Publication date
Publication date
Date available
Date available
Date available
ISSN or e-ISSN
ISSN or e-ISSN
ISSN or e-ISSN
OA Status
OA Status
OA Status
Publisher DOI
Metrics
Downloads
Views
Citations
Zini, E., Franchini, M., Guscetti, F., Osto, M., Kaufmann, K., Ackermann, M., Lutz, T. A., & Reusch, C. E. (2009). Assessment of six different collagenase-based methods to isolate feline pancreatic islets. Research in Veterinary Science, 87(3), 367–372. https://doi.org/10.1016/j.rvsc.2009.03.017
