Publication: RPA alleviates the inhibitory effect of vinylphosphonate internucleotide linkages on DNA unwinding by BLM and WRN helicases
RPA alleviates the inhibitory effect of vinylphosphonate internucleotide linkages on DNA unwinding by BLM and WRN helicases
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Garcia, P. L., Bradley, G., Hayes, C. H., Krintel, S. J., Soultanas, P., & Janscak, P. (2004). RPA alleviates the inhibitory effect of vinylphosphonate internucleotide linkages on DNA unwinding by BLM and WRN helicases. Nucleic Acids Research, 32, 3771–3778. https://doi.org/10.1093/nar/gkh709
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Abstract
Bloom (BLM) and Werner (WRN) syndrome proteins are members of the RecQ family of SF2 DNA helicases. In this paper, we show that restricting the rotational DNA backbone flexibility, by introducing vinylphosphonate internucleotide linkages in the translocating DNA strand, inhibits efficient duplex unwinding by these enzymes. The human single-stranded DNA binding protein replication protein A (RPA) fully restores the unwinding activity of BLM and WRN on vinylphosphonate-containing substrates while the heterologous single-stranded DNA bin
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- Garcia, P L
- Bradley, G
- Soultanas, P
- Janscak, Pavel
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Garcia, P. L., Bradley, G., Hayes, C. H., Krintel, S. J., Soultanas, P., & Janscak, P. (2004). RPA alleviates the inhibitory effect of vinylphosphonate internucleotide linkages on DNA unwinding by BLM and WRN helicases. Nucleic Acids Research, 32, 3771–3778. https://doi.org/10.1093/nar/gkh709
