Publication:

A New, Modular Mass Calibrant for High-Mass MALDI-MS

Date

Date

Date
2013
Journal Article
Published version

Citations

Citation copied

Weidmann, S., Barylyuk, K., Nespovitaya, N., Mädler, S., & Zenobi, R. (2013). A New, Modular Mass Calibrant for High-Mass MALDI-MS. Analytical Chemistry, 85, 3425–3432. https://doi.org/10.1021/ac400129h

Abstract

Abstract

Abstract

The application of matrix-assisted laser desorption/ionization mass spectrometry (MALDIMS) for the analysis of high-mass proteins requires suitable calibration standards at high m/z ratios. Several possible candidates were investigated, and concatenated polyproteins based on recombinantly expressed maltodextrin-binding protein (MBP) are shown here to be well suited for this purpose. Introduction of two specific recognition sites into the primary sequence of the polyprotein allows for the selective cleavage of MBP3 into MBP and MBP2. M

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133 since deposited on 2013-06-24
Acq. date: 2025-11-13

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129 since deposited on 2013-06-24
Acq. date: 2025-11-13

Additional indexing

Creators (Authors)

  • Weidmann, Simon
    affiliation.icon.alt
  • Barylyuk, Konstantin
    affiliation.icon.alt
  • Nespovitaya, Nadezhda
    affiliation.icon.alt
  • Mädler, Stefanie
    affiliation.icon.alt
  • Zenobi, Renato
    affiliation.icon.alt

Journal/Series Title

Journal/Series Title

Journal/Series Title

Volume

Volume

Volume
85

Page range/Item number

Page range/Item number

Page range/Item number
3425

Page end

Page end

Page end
3432

Item Type

Item Type

Item Type
Journal Article

In collections

Dewey Decimal Classifikation

Dewey Decimal Classifikation

Dewey Decimal Classifikation

Language

Language

Language
English

Publication date

Publication date

Publication date
2013-02

Date available

Date available

Date available
2013-06-24

Publisher

Publisher

Publisher

ISSN or e-ISSN

ISSN or e-ISSN

ISSN or e-ISSN
0003-2700

OA Status

OA Status

OA Status
Green

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Metrics

Downloads

133 since deposited on 2013-06-24
Acq. date: 2025-11-13

Views

129 since deposited on 2013-06-24
Acq. date: 2025-11-13

Citations

Citation copied

Weidmann, S., Barylyuk, K., Nespovitaya, N., Mädler, S., & Zenobi, R. (2013). A New, Modular Mass Calibrant for High-Mass MALDI-MS. Analytical Chemistry, 85, 3425–3432. https://doi.org/10.1021/ac400129h

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