Publication: Genome-scale CRISPR screening in human intestinal organoids identifies drivers of TGF-β resistance
Genome-scale CRISPR screening in human intestinal organoids identifies drivers of TGF-β resistance
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Ringel, T., Frey, N., Ringnalda, F., Janjuha, S., Cherkaoui, S., Butz, S., Srivatsa, S., Pirkl, M., Russo, G., Villiger, L., Rogler, G., Clevers, H., Beerenwinkel, N., Zamboni, N., Baubec, T., & Schwank, G. (2020). Genome-scale CRISPR screening in human intestinal organoids identifies drivers of TGF-β resistance. Cell Stem Cell, 26(3), 431-440.e8. https://doi.org/10.1016/j.stem.2020.02.007
Abstract
Abstract
Abstract
Forward genetic screens with genome-wide CRISPR libraries are powerful tools for resolving cellular circuits and signaling pathways. Applying this technology to organoids, however, has been hampered by technical limitations. Here we report improved accuracy and robustness for pooled-library CRISPR screens by capturing sgRNA integrations in single organoids, substantially reducing required cell numbers for genome-scale screening. We applied our approach to wild-type and APC mutant human intestinal organoids to identify genes involved i
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Creators (Authors)
- Ringel, Till
- Frey, Nina
- Ringnalda, Femke
- Janjuha, Sharan
- Cherkaoui, Sarah
- Butz, Stefan
- Srivatsa, Sumana
- Pirkl, Martin
- Russo, Giancarlo
- Villiger, Lukas
- Rogler, Gerhard
- Clevers, Hans
- Beerenwinkel, Niko
- Zamboni, Nicola
- Baubec, Tuncay
- Schwank, Gerald
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Citations
Ringel, T., Frey, N., Ringnalda, F., Janjuha, S., Cherkaoui, S., Butz, S., Srivatsa, S., Pirkl, M., Russo, G., Villiger, L., Rogler, G., Clevers, H., Beerenwinkel, N., Zamboni, N., Baubec, T., & Schwank, G. (2020). Genome-scale CRISPR screening in human intestinal organoids identifies drivers of TGF-β resistance. Cell Stem Cell, 26(3), 431-440.e8. https://doi.org/10.1016/j.stem.2020.02.007
