Publication: Isolation and characterization of equine uterine extracellular vesicles: a comparative methodological study
Isolation and characterization of equine uterine extracellular vesicles: a comparative methodological study
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Almiñana, C., Rudolf Vegas, A., Tekin, M., Hassan, M., Uzbekov, R., Fröhlich, T., Bollwein, H., & Bauersachs, S. (2021). Isolation and characterization of equine uterine extracellular vesicles: a comparative methodological study. International Journal of Molecular Sciences, 22(2), 979. https://doi.org/10.3390/ijms22020979
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Abstract
Extracellular vesicles (EVs) have been identified in the uterine fluid in different species and have been pointed as key players in the embryo-maternal dialogue, maternal recognition of pregnancy and establishment of pregnancy. However, little is known about the uterine EVs in the mare. Therefore, the present study aimed at characterizing EVs from uterine lavage of cyclic mares by comparing five EVs isolation methods and the combination of them: (1) ultracentrifugation (UC); (2) concentration of lavage volume by Centricon ultrafiltrat
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- Almiñana, Carmen
- Rudolf Vegas, Alba
- Tekin, Muhittin
- Hassan, Mubbashar
- Uzbekov, Rustem
- Fröhlich, Thomas
- Bollwein, Heiner
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Almiñana, C., Rudolf Vegas, A., Tekin, M., Hassan, M., Uzbekov, R., Fröhlich, T., Bollwein, H., & Bauersachs, S. (2021). Isolation and characterization of equine uterine extracellular vesicles: a comparative methodological study. International Journal of Molecular Sciences, 22(2), 979. https://doi.org/10.3390/ijms22020979
