Publication: Fluorescence Lifetime Multiplexing with Environment‐Sensitive Chemigenetic Probes
Fluorescence Lifetime Multiplexing with Environment‐Sensitive Chemigenetic Probes
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Emmert, S., Rovira, A., & Rivera Fuentes, P. (2025). Fluorescence Lifetime Multiplexing with Environment‐Sensitive Chemigenetic Probes. Chembiochem, 26(12), e202500174. https://doi.org/10.1002/cbic.202500174
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HaloTag (HT) is a versatile self‐labeling protein that has been widely adopted in fluorescence microscopy. Besides its established use as an intensity‐based marker and sensor, interest in using HT for multiplexed fluorescence lifetime imaging microscopy (FLIM) has recently arisen. Herein, the application of an environment‐sensitive fluorophore was explored for FLIM multiplexing with the free dye and HT mutants. The extended coumarin pyridinium scaffold was selected due to its structural simplicity and the strong sensitivity of its pho
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Emmert, S., Rovira, A., & Rivera Fuentes, P. (2025). Fluorescence Lifetime Multiplexing with Environment‐Sensitive Chemigenetic Probes. Chembiochem, 26(12), e202500174. https://doi.org/10.1002/cbic.202500174
