Publication: Expression and biophysical analysis of two double transmembrane domain-containing fragments from a yeast G protein-coupled receptor
Expression and biophysical analysis of two double transmembrane domain-containing fragments from a yeast G protein-coupled receptor
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Cohen, L. S., Arshava, B., Estephan, R., Englander, J., Kim, H., Hauser, M., Zerbe, O., Ceruso, M., Becker, J. M., & Naider, F. (2008). Expression and biophysical analysis of two double transmembrane domain-containing fragments from a yeast G protein-coupled receptor. Biopolymers, 90(2), 117–130. https://doi.org/10.1002/bip.20950
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Abstract
Fragments of G protein-coupled receptors (GPCRs) are widely used as models to investigate these polytopic integral–membrane, signal-transducing molecules but have proven difficult to prepare in quantities necessary for NMR analyses. We report on the biosynthesis of two double transmembrane (TM) containing fragments of Ste2p, the - factor GPCR from the yeast Saccharomyces cerevisiae. Ste2p(G31-G110) [TM1-TM2] and Ste2p(A231-S339) [TM6-TM7-CT40] were expressed as TrpLE fusion proteins in Escherichia coli and released by CNBr cleavage.
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- Cohen, L S
- Arshava, B
- Estephan, R
- Englander, J
- Kim, H
- Hauser, M
- Zerbe, O
- Ceruso, M
- Becker, J M
- Naider, F
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Cohen, L. S., Arshava, B., Estephan, R., Englander, J., Kim, H., Hauser, M., Zerbe, O., Ceruso, M., Becker, J. M., & Naider, F. (2008). Expression and biophysical analysis of two double transmembrane domain-containing fragments from a yeast G protein-coupled receptor. Biopolymers, 90(2), 117–130. https://doi.org/10.1002/bip.20950
