Publication: TimerQuant: a modelling approach to tandem fluorescent timer design and data interpretation for measuring protein turnover in embryos
TimerQuant: a modelling approach to tandem fluorescent timer design and data interpretation for measuring protein turnover in embryos
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Barry, J. D., Donà, E., Gilmour, D., & Huber, W. (2016). TimerQuant: a modelling approach to tandem fluorescent timer design and data interpretation for measuring protein turnover in embryos. Development, 143(1), 174–179. https://doi.org/10.1242/dev.125971
Abstract
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Abstract
Studies on signalling dynamics in living embryos have been limited by a scarcity of in vivo reporters. Tandem fluorescent protein timers provide a generic method for detecting changes in protein population age and thus provide readouts for signalling events that lead to changes in protein stability or location. When imaged with quantitative dual-colour fluorescence microscopy, tandem timers offer detailed 'snapshot' readouts of signalling activity from subcellular to organismal scales, and therefore have the potential to revolutionise
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- Barry, Joseph D
- Donà, Erika
- Huber, Wolfgang
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Citations
Barry, J. D., Donà, E., Gilmour, D., & Huber, W. (2016). TimerQuant: a modelling approach to tandem fluorescent timer design and data interpretation for measuring protein turnover in embryos. Development, 143(1), 174–179. https://doi.org/10.1242/dev.125971
