Publication: Broad-range 16S rRNA gene polymerase chain reaction for diagnosis of culture-negative bacterial infections
Broad-range 16S rRNA gene polymerase chain reaction for diagnosis of culture-negative bacterial infections
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Rampini, S. K., Bloemberg, G. V., Keller, P. M., Büchler, A. C., Dollenmaier, G., Speck, R. F., & Böttger, E. C. (2011). Broad-range 16S rRNA gene polymerase chain reaction for diagnosis of culture-negative bacterial infections. Clinical Infectious Diseases, 53(12), 1245–1251. https://doi.org/10.1093/cid/cir692
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BACKGROUND:
Broad-range 16S ribosomal RNA (rRNA) gene polymerase chain reaction (PCR) is used for detection and identification of bacterial pathogens in clinical specimens from patients with a high suspicion for infection. However, prospective studies addressing the impact and clinical value of broad-range bacterial 16S rRNA gene amplification for diagnosis of acute infectious diseases in nonselected patient populations are lacking. METHODS:
We first assessed the diagnostic performance of 16S rRNA gene PCR compared with routine bact
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- Rampini, S K
- Bloemberg, G V
- Keller, P M
- Büchler, A C
- Dollenmaier, G
- Speck, R F
- Böttger, E C
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Rampini, S. K., Bloemberg, G. V., Keller, P. M., Büchler, A. C., Dollenmaier, G., Speck, R. F., & Böttger, E. C. (2011). Broad-range 16S rRNA gene polymerase chain reaction for diagnosis of culture-negative bacterial infections. Clinical Infectious Diseases, 53(12), 1245–1251. https://doi.org/10.1093/cid/cir692
