Genotyping-by-sequencing-based identification of Arabidopsis pattern recognition receptor RLP32 recognizing proteobacterial translation initiation factor IF1

Fan, Li; Fröhlich, Katja; Melzer, Eric; Pruitt, Rory N; Albert, Isabell; Zhang, Lisha; Joe, Anna; Hua, Chenlei; Song, Yanyue; Albert, Markus; Kim, Sang-Tae; Weigel, Detlef; Zipfel, Cyril; Chae, Eunyoung; Gust, Andrea A; Nürnberger, Thorsten

doi:10.5167/uzh-218106

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Fan, L., Fröhlich, K., Melzer, E., Pruitt, R. N., Albert, I., Zhang, L., Joe, A., Hua, C., Song, Y., Albert, M., Kim, S.-T., Weigel, D., Zipfel, C., Chae, E., Gust, A. A., & Nürnberger, T. (2022). Genotyping-by-sequencing-based identification of Arabidopsis pattern recognition receptor RLP32 recognizing proteobacterial translation initiation factor IF1. Nature Communications, 13, 1294. https://doi.org/10.1038/s41467-022-28887-4

Abstract

Activation of plant pattern-triggered immunity (PTI) relies on the recognition of microbe-derived structures, termed patterns, through plant-encoded surface-resident pattern recognition receptors (PRRs). We show that proteobacterial translation initiation factor 1 (IF1) triggers PTI in Arabidopsis thaliana and related Brassicaceae species. Unlike for most other immunogenic patterns, IF1 elicitor activity cannot be assigned to a small peptide epitope, suggesting that tertiary fold features are required for IF1 receptor activation. We h