Publication: The yeast expression system for recombinant glycosyltransferases
The yeast expression system for recombinant glycosyltransferases
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Malissard, M., Zeng, S., & Berger, E. G. (1999). The yeast expression system for recombinant glycosyltransferases. Glycoconjugate Journal, 16(2), 125–139. https://doi.org/10.1023/a:1007055525789
Abstract
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Abstract
Glycosyltransferases are increasingly being used for in vitro synthesis of oligosaccharides. Since these enzymes are difficult to purify from natural sources, expression systems for soluble forms of the recombinant enzymes have been developed. This review focuses on the current state of development of yeast expression systems. Two yeast species have mainly been used, i.e. Saccharomyces cerevisiae and Pichia pastoris. Safety and ease of fermentation are well recognized for S. cerevisiae as a biotechnological expression system; however,
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- Malissard, Martine
- Zeng, Steffen
- Berger, Eric G
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Malissard, M., Zeng, S., & Berger, E. G. (1999). The yeast expression system for recombinant glycosyltransferases. Glycoconjugate Journal, 16(2), 125–139. https://doi.org/10.1023/a:1007055525789
