Publication:

Injection and electroporation of plasmid DNA into human cortical organoids

Date

Date

Date
2022
Journal Article
Published version
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cris.lastimport.scopus2025-06-20T03:34:42Z
dc.contributor.institutionUniversity of Zurich
dc.date.accessioned2023-02-24T12:48:44Z
dc.date.available2023-02-24T12:48:44Z
dc.date.issued2022-03-18
dc.description.abstract

Pluripotent stem cell-derived human cortical organoids allow for the analysis of stem cell behavior and neurogenesis in neural tissues. Delivery of plasmid DNA into organoids permits visualization of individual cells, characterization of cellular components, and manipulation of gene expression. We describe a protocol to transfect cells inside organoids with plasmid DNA using micro-injection and electroporation, allowing for DNA delivery to cells within cortical units. This protocol was optimized for cortical organoids; however, it may be adapted to other organoid models. For complete details on the use and execution of this protocol, please refer to Denoth-Lippuner et al. (2021).

dc.identifier.doi10.1016/j.xpro.2022.101129
dc.identifier.issn2666-1667
dc.identifier.scopus2-s2.0-85123096551
dc.identifier.urihttps://www.zora.uzh.ch/handle/20.500.14742/206298
dc.language.isoeng
dc.subject.ddc570 Life sciences; biology
dc.subject.ddc610 Medicine & health
dc.title

Injection and electroporation of plasmid DNA into human cortical organoids

dc.typearticle
dcterms.accessRightsinfo:eu-repo/semantics/openAccess
dcterms.bibliographicCitation.journaltitleSTAR Protocols
dcterms.bibliographicCitation.number1
dcterms.bibliographicCitation.originalpublishernameCell Press (Elsevier)
dcterms.bibliographicCitation.pagestart101129
dcterms.bibliographicCitation.pmid35118433
dcterms.bibliographicCitation.volume3
dspace.entity.typePublicationen
uzh.contributor.affiliationUniversity of Zurich
uzh.contributor.affiliationUniversity of Zurich
uzh.contributor.affiliationUniversity of Zurich
uzh.contributor.affiliationUniversity of Zurich
uzh.contributor.affiliationUniversity of Zurich
uzh.contributor.authorDenoth-Lippuner, Annina
uzh.contributor.authorRoyall, Lars N
uzh.contributor.authorGonzalez-Bohorquez, Daniel
uzh.contributor.authorMachado, Diana
uzh.contributor.authorJessberger, Sebastian
uzh.contributor.correspondenceYes
uzh.contributor.correspondenceNo
uzh.contributor.correspondenceNo
uzh.contributor.correspondenceNo
uzh.contributor.correspondenceYes
uzh.document.availabilitypublished_version
uzh.eprint.datestamp2023-02-24 12:48:44
uzh.eprint.lastmod2025-06-20 03:34:42
uzh.eprint.statusChange2023-02-24 12:48:44
uzh.harvester.ethYes
uzh.harvester.nbNo
uzh.identifier.doi10.5167/uzh-231745
uzh.jdb.eprintsId44452
uzh.oastatus.unpaywallgold
uzh.oastatus.zoraGold
uzh.publication.citationDenoth-Lippuner, Annina; Royall, Lars N; Gonzalez-Bohorquez, Daniel; Machado, Diana; Jessberger, Sebastian (2022). Injection and electroporation of plasmid DNA into human cortical organoids. STAR Protocols, 3(1):101129.
uzh.publication.freeAccessAtpubmedid
uzh.publication.originalworkoriginal
uzh.publication.publishedStatusfinal
uzh.scopus.impact4
uzh.scopus.subjectsGeneral Biochemistry, Genetics and Molecular Biology
uzh.scopus.subjectsGeneral Neuroscience
uzh.scopus.subjectsGeneral Immunology and Microbiology
uzh.scopus.subjectsGeneral Medicine
uzh.workflow.doajuzh.workflow.doaj.true
uzh.workflow.eprintid231745
uzh.workflow.fulltextStatuspublic
uzh.workflow.revisions21
uzh.workflow.rightsCheckoffen
uzh.workflow.sourcePubMed:PMID:35118433
uzh.workflow.statusarchive
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