Production and Characterization of Peptide Antibodies to the C-Terminal of Frameshifted Calreticulin Associated with Myeloproliferative Diseases

Mughal, Farah Perveen; Bergmann, Ann Christina; Huynh, Ha Uyen Buu; Jørgensen, Sarah Hyllekvist; Mansha, Inaam; Kesmez, Meliha; Schürch, Patrick Mark; Theocharides, Alexandre Pierre André; Hansen, Paul Robert; Friis, Tina; Holmström, Morten Orebo; Ciplys, Evaldas; Slibinskas, Rimantas; Højrup, Peter; Houen, Gunnar; Trier, Nicole Hartwig

doi:10.3390/ijms23126803

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Mughal, F. P., Bergmann, A. C., Huynh, H. U. B., Jørgensen, S. H., Mansha, I., Kesmez, M., Schürch, P. M., Theocharides, A. P. A., Hansen, P. R., Friis, T., Holmström, M. O., Ciplys, E., Slibinskas, R., Højrup, P., Houen, G., & Trier, N. H. (2022). Production and Characterization of Peptide Antibodies to the C-Terminal of Frameshifted Calreticulin Associated with Myeloproliferative Diseases. International Journal of Molecular Sciences, 23(12), 6803. https://doi.org/10.3390/ijms23126803

Abstract

Myeloproliferative Neoplasms (MPNs) constitute a group of rare blood cancers that are characterized by mutations in bone marrow stem cells leading to the overproduction of erythrocytes, leukocytes, and thrombocytes. Mutations in calreticulin (CRT) genes may initiate MPNs, causing a novel variable polybasic stretch terminating in a common C-terminal sequence in the frameshifted CRT (CRTfs) proteins. Peptide antibodies to the mutated C-terminal are important reagents for research in the molecular mechanisms of MPNs and for the developme