A transcriptomics resource reveals a transcriptional transition during ordered sarcomere morphogenesis in flight muscle

Spletter, Maria L; Barz, Christiane; Yeroslaviz, Assa; Zhang, Xu; Lemke, Sandra B; Bonnard, Adrien; Brunner, Erich; Cardone, Giovanni; Basler, Konrad; Habermann, Bianca H; Schnorrer, Frank

doi:10.7554/eLife.34058

Citations

Citation copied

Spletter, M. L., Barz, C., Yeroslaviz, A., Zhang, X., Lemke, S. B., Bonnard, A., Brunner, E., Cardone, G., Basler, K., Habermann, B. H., & Schnorrer, F. (2018). A transcriptomics resource reveals a transcriptional transition during ordered sarcomere morphogenesis in flight muscle. ELife, 7, e34058. https://doi.org/10.7554/eLife.34058

Abstract

Muscles organise pseudo-crystalline arrays of actin, myosin and titin filaments to build force-producing sarcomeres. To study sarcomerogenesis, we have generated a transcriptomics resource of developing flight muscles and identified 40 distinct expression profile clusters. Strikingly, most sarcomeric components group in two clusters, which are strongly induced after all myofibrils have been assembled, indicating a transcriptional transition during myofibrillogenesis. Following myofibril assembly, many short sarcomeres are added to ea