Three-dimensional wide-field fluorescence microscopy for transcranial mapping of cortical microcirculation

Zhou, Quanyu; Chen, Zhenyue; Liu, Yu-Hang; El Amki, Mohamad; Glück, Chaim; Droux, Jeanne; Reiss, Michael V; Weber, Bruno; Wegener, Susanne; Razansky, Daniel

doi:10.1038/s41467-022-35733-0

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Zhou, Q., Chen, Z., Liu, Y.-H., El Amki, M., Glück, C., Droux, J., Reiss, M. V., Weber, B., Wegener, S., & Razansky, D. (2022). Three-dimensional wide-field fluorescence microscopy for transcranial mapping of cortical microcirculation. Nature Communications, 13(1), 7969. https://doi.org/10.1038/s41467-022-35733-0

Abstract

Wide-field fluorescence imaging is an indispensable tool for studying large-scale biodynamics. Limited space-bandwidth product and strong light diffusion make conventional implementations incapable of high-resolution mapping of fluorescence biodistribution in three dimensions. We introduce a volumetric wide-field fluorescence microscopy based on optical astigmatism combined with fluorescence source localization, covering 5.6×5.6×0.6 mm3 imaging volume. Two alternative configurations are proposed exploiting multifocal illumination or s