CRHP Finder, a webtool for the detection of clarithromycin resistance in Helicobacter pylori from whole-genome sequencing data

Yusibova, Melodi; Hasman, Henrik; Clausen, Philip Thomas Lanken Conradsen; Imkamp, Frank; Wagner, Karoline; Andersen, Leif Percival

doi:10.1111/hel.12752

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Yusibova, M., Hasman, H., Clausen, P. T. L. C., Imkamp, F., Wagner, K., & Andersen, L. P. (2020). CRHP Finder, a webtool for the detection of clarithromycin resistance in Helicobacter pylori from whole-genome sequencing data. Helicobacter, 25, e12752. https://doi.org/10.1111/hel.12752

Abstract

BACKGROUND

Resistance to clarithromycin in Helicobacter pylori (H pylori) is mediated by mutations in the domain V of the 23S rRNA gene (A2142G, A2143G, A2142C). Other polymorphisms in the 23S rRNA gene have been reported to cause low-level clarithromycin resistance but their importance is still under debate. In this study, we aimed to develop and evaluate the CRHP Finder webtool for detection of the most common mutations mediating clarithromycin resistance from whole-genome sequencing (WGS) data. Moreover, we included an analysis of