Publication: Microfluidics-based immunofluorescence for fast staining of ALK in lung adenocarcinoma
Microfluidics-based immunofluorescence for fast staining of ALK in lung adenocarcinoma
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Brajkovic, S., Pelz, B., Procopio, M.-G., Leblond, A.-L., Repond, G., Schaub-Clerigué, A., Dupouy, D. G., & Soltermann, A. (2018). Microfluidics-based immunofluorescence for fast staining of ALK in lung adenocarcinoma. Diagnostic Pathology, 13, 79. https://doi.org/10.1186/s13000-018-0757-1
Abstract
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Abstract
BACKGROUND Anaplastic lymphoma kinase (ALK) is a key oncogenic driver in lung adenocarcinoma patients and its fusion proteins are routinely assessed. The microfluidic tissue processor (MTP) device is based on a chip-confined low-volume technology allowing for rapid immunohistochemistry/immunofluorescence (IHC/IF) stainings of formalin-fixed paraffin-embedded (FFPE) or frozen tissue samples. METHODS A novel ALK IF protocol was developed for the MTP device using the primary mouse anti-human ALK antibody clone 5A4. FFPE tumor whole se
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- Brajkovic, Saška
- Pelz, Benjamin
- Procopio, Maria-Giuseppina
- Leblond, Anne-Laure
- Repond, Grégoire
- Schaub-Clerigué, Ariane
- Dupouy, Diego G
- Soltermann, Alex
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Brajkovic, S., Pelz, B., Procopio, M.-G., Leblond, A.-L., Repond, G., Schaub-Clerigué, A., Dupouy, D. G., & Soltermann, A. (2018). Microfluidics-based immunofluorescence for fast staining of ALK in lung adenocarcinoma. Diagnostic Pathology, 13, 79. https://doi.org/10.1186/s13000-018-0757-1
