The Legionella longbeachae Icm/Dot substrate SidC selectively binds phosphatidylinositol 4-phosphate with nanomolar affinity and promotes pathogen vacuole-endoplasmic reticulum interactions

Dolinsky, Stephanie; Haneburger, Ina; Cichy, Adam; Hannemann, Mandy; Itzen, Aymelt; Hilbi, Hubert

doi:10.1128/IAI.01685-14

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Dolinsky, S., Haneburger, I., Cichy, A., Hannemann, M., Itzen, A., & Hilbi, H. (2014). The Legionella longbeachae Icm/Dot substrate SidC selectively binds phosphatidylinositol 4-phosphate with nanomolar affinity and promotes pathogen vacuole-endoplasmic reticulum interactions. Infection and Immunity, 82(10), 4021–4033. https://doi.org/10.1128/IAI.01685-14

Abstract

Legionella spp. cause the severe pneumonia Legionnaires' disease. The environmental bacteria replicate intracellularly in free-living amoebae and human alveolar macrophages within a distinct, endoplasmic reticulum (ER)-derived compartment termed the Legionella-containing vacuole (LCV). LCV formation requires the bacterial Icm/Dot type IV secretion system (T4SS) that translocates into host cells a plethora of different "effector" proteins, some of which anchor to the pathogen vacuole by binding to phosphoinositide (PI) lipids. Here, we