Publication: Reverse Genetic Assessment of the Roles Played by the Spike Protein and ORF3 in Porcine Epidemic Diarrhea Virus Pathogenicity
Reverse Genetic Assessment of the Roles Played by the Spike Protein and ORF3 in Porcine Epidemic Diarrhea Virus Pathogenicity
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Kristen-Burmann, C., Rogger, P., Veiga, I. B., Riebesehl, S., Rappe, J., Ebert, N., Sautter, C. A., Kelly, J. N., Stalder, H., Ehmann, R., Huber, M., Posthaus, H., Ruggli, N., Thiel, V., & Tekes, G. (2023). Reverse Genetic Assessment of the Roles Played by the Spike Protein and ORF3 in Porcine Epidemic Diarrhea Virus Pathogenicity. Journal of Virology, 97(7), e0196422. https://doi.org/10.1128/jvi.01964-22
Abstract
Abstract
Abstract
Porcine epidemic diarrhea virus is a swine pathogen that has been responsible for significant animal and economic losses worldwide in recent years. In this manuscript, we report the generation of a reverse genetics system C(RGS) for the highly virulent US PEDV strain Minnesota (PEDV-MN; GenBank accession number KF468752), which was based on the assembly and cloning of synthetic DNA, using vaccinia virus as a cloning vector. Viral rescue was only possible following the substitution of 2 nucleotides within the 5'UTR and 2 additional nuc
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Creators (Authors)
- Kristen-Burmann, Claudia
- Rogger, Peter
- Veiga, Inês Berenguer
- Riebesehl, Stefanie
- Rappe, Julie
- Ebert, Nadine
- Sautter, Carmen A
- Kelly, Jenna N
- Stalder, Hanspeter
- Ehmann, Rosina
- Posthaus, Horst
- Ruggli, Nicolas
- Thiel, Volker
- Tekes, Gergely
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Citations
Kristen-Burmann, C., Rogger, P., Veiga, I. B., Riebesehl, S., Rappe, J., Ebert, N., Sautter, C. A., Kelly, J. N., Stalder, H., Ehmann, R., Huber, M., Posthaus, H., Ruggli, N., Thiel, V., & Tekes, G. (2023). Reverse Genetic Assessment of the Roles Played by the Spike Protein and ORF3 in Porcine Epidemic Diarrhea Virus Pathogenicity. Journal of Virology, 97(7), e0196422. https://doi.org/10.1128/jvi.01964-22
