The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue

Voigt, Fabian F; Kirschenbaum, Daniel; Platonova, Evgenia; Pagès, Stéphane; Campbell, Robert A A; Kastli, Rahel; Schaettin, Martina; Egolf, Ladan; van der Bourg, Alexander; Bethge, Philipp; Haenraets, Karen; Frézel, Noémie; Topilko, Thomas; Perin, Paola; Hillier, Daniel; Hildebrand, Sven; Schueth, Anna; Roebroeck, Alard; Roska, Botond; Stoeckli, Esther T

doi:10.1038/s41592-019-0554-0

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Voigt, F. F., Kirschenbaum, D., Platonova, E., Pagès, S., Campbell, R. A. A., Kastli, R., Schaettin, M., Egolf, L., van der Bourg, A., Bethge, P., Haenraets, K., Frézel, N., Topilko, T., Perin, P., Hillier, D., Hildebrand, S., Schueth, A., Roebroeck, A., Roska, B., … Helmchen, F. (2019). The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue. Nature Methods, 16(11), 1105–1108. https://doi.org/10.1038/s41592-019-0554-0

Abstract

Light-sheet microscopy is an ideal technique for imaging large cleared samples; however, the community is still lacking instruments capable of producing volumetric images of centimeter-sized cleared samples with near-isotropic resolution within minutes. Here, we introduce the mesoscale selective plane-illumination microscopy initiative, an open-hardware project for building and operating a light-sheet microscope that addresses these challenges and is compatible with any type of cleared or expanded sample ( www.mesospim.org ).