Long-term self-renewing stem cells in the adult mouse hippocampus identified by intravital imaging

Bottes, Sara; Jaeger, Baptiste N; Pilz, Gregor-Alexander; Jörg, David J; Cole, John Darby; Kruse, Merit; Harris, Lachlan; Korobeynyk, Vladislav I; Mallona, Izaskun; Helmchen, Fritjof; Guillemot, François; Simons, Benjamin D; Jessberger, Sebastian

doi:10.1038/s41593-020-00759-4

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Bottes, S., Jaeger, B. N., Pilz, G.-A., Jörg, D. J., Cole, J. D., Kruse, M., Harris, L., Korobeynyk, V. I., Mallona, I., Helmchen, F., Guillemot, F., Simons, B. D., & Jessberger, S. (2021). Long-term self-renewing stem cells in the adult mouse hippocampus identified by intravital imaging. Nature Neuroscience, 24, 225–233. https://doi.org/10.1038/s41593-020-00759-4

Abstract

Neural stem cells (NSCs) generate neurons throughout life in the mammalian hippocampus. However, the potential for long-term self-renewal of individual NSCs within the adult brain remains unclear. We used two-photon microscopy and followed NSCs that were genetically labeled through conditional recombination driven by the regulatory elements of the stem cell-expressed genes GLI family zinc finger 1 (Gli1) or achaete-scute homolog 1 (Ascl1). Through intravital imaging of NSCs and their progeny, we identify a population of Gli1-targeted