Human Dermal Microvascular Arterial and Venous Blood Endothelial Cells and Their Use in Bioengineered Dermo‐Epidermal Skin Substitutes

Rütsche, Dominic; Nanni, Monica; Cheng, Phil; Caflisch, Nicolà; Tastanova, Aizhan; Jenni, Céline; Levesque, Mitchell P; Moehrlen, Ueli; Klar, Agnes S; Biedermann, Thomas

doi:10.1002/smtd.202401588

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Rütsche, D., Nanni, M., Cheng, P., Caflisch, N., Tastanova, A., Jenni, C., Levesque, M. P., Moehrlen, U., Klar, A. S., & Biedermann, T. (2025). Human Dermal Microvascular Arterial and Venous Blood Endothelial Cells and Their Use in Bioengineered Dermo‐Epidermal Skin Substitutes. Small Methods, Epub ahead of print. https://doi.org/10.1002/smtd.202401588

Abstract

The bioengineering of vascular networks is pivotal to create complex tissues and organs for regenerative medicine applications. However, bioengineered tissues comprising an arterial and venous plexus alongside a lymphatic capillary network have not been explored yet. Here, scRNA‐seq is first employed to investigate the arterio‐venous endothelial cell marker patterning in human fetal and juvenile skin. Transcriptomic analysis reveals that arterial and venous endothelial cell markers NRP1 (neuropilin 1) and NR2F2 (nuclear receptor subfa