Asynchronous embryo transfer followed by comparative transcriptomic analysis of conceptus membranes and endometrium identifies processes important to the establishment of equine pregnancy

Gibson, Charlotte; de Ruijter-Villani, Marta; Bauersachs, Stefan; Stout, Tom A E

doi:10.3390/ijms21072562

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Gibson, C., de Ruijter-Villani, M., Bauersachs, S., & Stout, T. A. E. (2020). Asynchronous embryo transfer followed by comparative transcriptomic analysis of conceptus membranes and endometrium identifies processes important to the establishment of equine pregnancy. International Journal of Molecular Sciences, 21(7), 2562. https://doi.org/10.3390/ijms21072562

Abstract

Preimplantation horse conceptuses require nutrients and signals from histotroph, the composition of which is regulated by luteal progesterone and conceptus-secreted factors. To distinguish progesterone and conceptus effects we shortened the period of endometrial progesterone-priming by asynchronous embryo transfer. Day 8 embryos were transferred to synchronous (day 8) or asynchronous (day 3) recipients, and RNA sequencing was performed on endometrium and conceptuses recovered 6 and 11 days later (embryo days 14 and 19). Asynchrony res