Publication: FRaeppli: a multispectral imaging toolbox for cell tracing and dense tissue analysis in zebrafish
FRaeppli: a multispectral imaging toolbox for cell tracing and dense tissue analysis in zebrafish
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Caviglia, S., Unterweger, I. A., Gasiūnaitė, A., Vanoosthuyse, A. E., Cutrale, F., Trinh, L. A., Fraser, S. E., Neuhauss, S. C. F., & Ober, E. A. (2022). FRaeppli: a multispectral imaging toolbox for cell tracing and dense tissue analysis in zebrafish. Development, 149(16), dev199615. https://doi.org/10.1242/dev.199615
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Abstract
Visualizing cell shapes and interactions of differentiating cells is instrumental for understanding organ development and repair. Across species, strategies for stochastic multicolour labelling have greatly facilitated in vivo cell tracking and mapping neuronal connectivity. Yet integrating multi-fluorophore information into the context of developing zebrafish tissues is challenging given their cytoplasmic localization and spectral incompatibility with common fluorescent markers. Inspired by Drosophila Raeppli, we developed FRaeppli (
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- Unterweger, Iris A
- Gasiūnaitė, Akvilė
- Vanoosthuyse, Alexandre E
- Cutrale, Francesco
- Trinh, Le A
- Fraser, Scott E
- Ober, Elke A
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Caviglia, S., Unterweger, I. A., Gasiūnaitė, A., Vanoosthuyse, A. E., Cutrale, F., Trinh, L. A., Fraser, S. E., Neuhauss, S. C. F., & Ober, E. A. (2022). FRaeppli: a multispectral imaging toolbox for cell tracing and dense tissue analysis in zebrafish. Development, 149(16), dev199615. https://doi.org/10.1242/dev.199615
