Publication: Negative effects of oxidative stress in bovine spermatozoa on in vitro development and DNA integrity of embryos
Negative effects of oxidative stress in bovine spermatozoa on in vitro development and DNA integrity of embryos
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Bittner, L., Wyck, S., Herrera, C., Siuda, M., Wrenzycki, C., van Loon, B., & Bollwein, H. (2018). Negative effects of oxidative stress in bovine spermatozoa on in vitro development and DNA integrity of embryos. Reproduction, Fertility and Development, 30(10), 1359–1368. https://doi.org/10.1071/rd17533
Abstract
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Abstract
Oxidative stress in spermatozoa has effects on subsequent embryo development. The aim of the present study was to elucidate whether sperm oxidative stress results in increased DNA damage in the embryo. To this end, bovine spermatozoa were incubated for 1 h at 37°C without or with 100 µM H2O2, resulting in non-oxidised (NOX-S) and oxidised (OX-S) spermatozoa respectively. Non-incubated spermatozoa served as the control group (CON-S). After IVF, developmental rates 30, 46 and 60 h and 7 days after IVF were assessed. DNA damage was analy
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- Bittner, L
- Wyck, S
- Herrera, C
- Siuda, M
- Wrenzycki, C
- van Loon, B
- Bollwein, Heiner
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Bittner, L., Wyck, S., Herrera, C., Siuda, M., Wrenzycki, C., van Loon, B., & Bollwein, H. (2018). Negative effects of oxidative stress in bovine spermatozoa on in vitro development and DNA integrity of embryos. Reproduction, Fertility and Development, 30(10), 1359–1368. https://doi.org/10.1071/rd17533
