Publication: Cell-wide analysis of protein thermal unfolding reveals determinants of thermostability
Cell-wide analysis of protein thermal unfolding reveals determinants of thermostability
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Leuenberger, P., Ganscha, S., Kahraman, A., Cappelletti, V., Boersema, P. J., von Mering, C., Claassen, M., & Picotti, P. (2017). Cell-wide analysis of protein thermal unfolding reveals determinants of thermostability. Science, 355, 1–13. https://doi.org/10.1126/science.aai7825
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Temperature-induced cell death is thought to be due to protein denaturation, but the determinants of thermal sensitivity of proteomes remain largely uncharacterized. We developed a structural proteomic strategy to measure protein thermostability on a proteome-wide scale and with domain-level resolution. We applied it to Escherichia coli, Saccharomyces cerevisiae, Thermus thermophilus, and human cells, yielding thermostability data for more than 8000 proteins. Our results (i) indicate that temperature-induced cellular collapse is due t
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- Leuenberger, Pascal
- Ganscha, Stefan
- Kahraman, Abdullah
- Cappelletti, Valentina
- Boersema, Paul J
- Claassen, Manfred
- Picotti, Paola
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Leuenberger, P., Ganscha, S., Kahraman, A., Cappelletti, V., Boersema, P. J., von Mering, C., Claassen, M., & Picotti, P. (2017). Cell-wide analysis of protein thermal unfolding reveals determinants of thermostability. Science, 355, 1–13. https://doi.org/10.1126/science.aai7825
