Publication: Elastic volume reconstruction from series of ultra-thin microscopy sections
Elastic volume reconstruction from series of ultra-thin microscopy sections
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Saalfeld, S., Fetter, R., Cardona, A., & Tomancak, P. (2012). Elastic volume reconstruction from series of ultra-thin microscopy sections. Nature Methods, 9(7), 717–720. https://doi.org/10.1038/nmeth.2072
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Anatomy of large biological specimens is often reconstructed from serially sectioned volumes imaged by high-resolution microscopy. We developed a method to reassemble a continuous volume from such large section series that explicitly minimizes artificial deformation by applying a global elastic constraint. We demonstrate our method on a series of transmission electron microscopy sections covering the entire 558-cell Caenorhabditis elegans embryo and a segment of the Drosophila melanogaster larval ventral nerve cord.
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- Saalfeld, Stephan
- Fetter, Richard
- Cardona, Albert
- Tomancak, Pavel
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Saalfeld, S., Fetter, R., Cardona, A., & Tomancak, P. (2012). Elastic volume reconstruction from series of ultra-thin microscopy sections. Nature Methods, 9(7), 717–720. https://doi.org/10.1038/nmeth.2072
