Bioengineering commensal bacteria-derived outer membrane vesicles for delivery of biologics to the gastrointestinal and respiratory tract

Carvalho, Ana L; Fonseca, Sonia; Miquel-Clopés, Ariadna; Cross, Kathryn; Kok, Khoon-S; Wegmann, Udo; Gil-Cardoso, Katherine; Bentley, Eleanor G; Al Katy, Sanaria H M; Coombes, Janine L; Kipar, Anja; Stentz, Regis; Stewart, James P; Carding, Simon R

doi:10.1080/20013078.2019.1632100

Citations

Citation copied

Carvalho, A. L., Fonseca, S., Miquel-Clopés, A., Cross, K., Kok, K.-S., Wegmann, U., Gil-Cardoso, K., Bentley, E. G., Al Katy, S. H. M., Coombes, J. L., Kipar, A., Stentz, R., Stewart, J. P., & Carding, S. R. (2019). Bioengineering commensal bacteria-derived outer membrane vesicles for delivery of biologics to the gastrointestinal and respiratory tract. Journal of Extracellular Vesicles, 8(1), 1632100. https://doi.org/10.1080/20013078.2019.1632100

Abstract

Gram-negative bacteria naturally produce and secrete nanosized outer membrane vesicles (OMVs). In the human gastrointestinal tract, OMVs produced by commensal Gram-negative bacteria can mediate interactions amongst host cells (including between epithelial cells and immune cells) and maintain microbial homeostasis. This OMV-mediated pathway for host-microbe interactions could be exploited to deliver biologically active proteins to the body. To test this we engineered the Gram-negative bacterium Bacteroides thetaiotaomicron (Bt), a prom