Transcatheter based electromechanical mapping guided intramyocardial transplantation and in vivo tracking of human stem cell based three dimensional microtissues in the porcine heart

Emmert, Maximilian Y; Wolint, Petra; Winklhofer, Sebastian; Stolzmann, Paul; Cesarovic, Nikola; Fleischmann, Thea; Nguyen, Thi D L; Frauenfelder, Thomas; Böni, Roland; Scherman, Jacques; Bettex, Dominique; Grünenfelder, Jürg; Schwartlander, Ruth; Vogel, Viola; Gyöngyösi, Mariann; Alkadhi, Hatem; Falk, Volkmar; Hoerstrup, Simon P

doi:10.1016/j.biomaterials.2012.12.021

Citations

Citation copied

Emmert, M. Y., Wolint, P., Winklhofer, S., Stolzmann, P., Cesarovic, N., Fleischmann, T., Nguyen, T. D. L., Frauenfelder, T., Böni, R., Scherman, J., Bettex, D., Grünenfelder, J., Schwartlander, R., Vogel, V., Gyöngyösi, M., Alkadhi, H., Falk, V., & Hoerstrup, S. P. (2013). Transcatheter based electromechanical mapping guided intramyocardial transplantation and in vivo tracking of human stem cell based three dimensional microtissues in the porcine heart. Biomaterials, 34(10), 2428–2441. https://doi.org/10.1016/j.biomaterials.2012.12.021

Abstract

Stem cells have been repeatedly suggested for cardiac regeneration after myocardial infarction (MI). However, the low retention rate of single cell suspensions limits the efficacy of current therapy concepts so far. Taking advantage of three dimensional (3D) cellular self-assembly prior to transplantation may be beneficial to overcome these limitations. In this pilot study we investigate the principal feasibility of intramyocardial delivery of in-vitro generated stem cell-based 3D microtissues (3D-MTs) in a porcine model. 3D-MTs were