Publication: Catalytic $pK_a$ Attenuation in a Hydrolytic Metalloenzyme by Genetic Code Expansion
Catalytic $pK_a$ Attenuation in a Hydrolytic Metalloenzyme by Genetic Code Expansion
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Manser, B. P., & Deliz Liang, A. (2026). Catalytic $pK_a$ Attenuation in a Hydrolytic Metalloenzyme by Genetic Code Expansion. Biochemistry, 65, 559–570. https://doi.org/10.1021/acs.biochem.5c00768
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Hydrolytic metalloenzymes employ Lewis-acidic metal cofactors to activate water molecules, generating nucleophilic hydroxide species that facilitate catalysis. Their catalytic efficiency across a wide pH range is often governed by the protonation state of the metal-bound water, reflected in pKa values typically between 6.8 and 9. Modulating this parameter is key to expanding enzymatic activity for improved activity at neutral to acidic pH. Herein, we apply genetic code expansion to mutate the primary metal-coordination sphere of a mod
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Manser, B. P., & Deliz Liang, A. (2026). Catalytic $pK_a$ Attenuation in a Hydrolytic Metalloenzyme by Genetic Code Expansion. Biochemistry, 65, 559–570. https://doi.org/10.1021/acs.biochem.5c00768
