Publication: Optimization of the culturing conditions of human umbilical cord blood-derived endothelial colony-forming cells under xeno-free conditions applying a transcriptomic approach
Optimization of the culturing conditions of human umbilical cord blood-derived endothelial colony-forming cells under xeno-free conditions applying a transcriptomic approach
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Zeisberger, S. M., Zoller, S., Riegel, M., Chen, S., Krenning, G., Harmsen, M. C., Sachinidis, A., & Zisch, A. H. (2010). Optimization of the culturing conditions of human umbilical cord blood-derived endothelial colony-forming cells under xeno-free conditions applying a transcriptomic approach. Genes to Cells, 15, 671–687. https://doi.org/10.1111/j.1365-2443.2010.01409.x
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Establishment of fetal bovine serum (FBS)-free cell culture conditions is essential for transplantation therapies. Blood-derived endothelial colony-forming cells (ECFCs) are potential candidates for regenerative medicine applications. ECFCs were isolated from term umbilical cord blood units and characterized by flow cytometry, capillary formation and responsiveness to cytokines. ECFCs were expanded under standard, FBS-containing endothelial medium, or transferred to chemically defined endothelial media without FBS. Microarray expressi
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Zeisberger, S. M., Zoller, S., Riegel, M., Chen, S., Krenning, G., Harmsen, M. C., Sachinidis, A., & Zisch, A. H. (2010). Optimization of the culturing conditions of human umbilical cord blood-derived endothelial colony-forming cells under xeno-free conditions applying a transcriptomic approach. Genes to Cells, 15, 671–687. https://doi.org/10.1111/j.1365-2443.2010.01409.x