Acellular urethra bioscaffold: decellularization of whole urethras for tissue engineering applications

Simões, Irina N; Vale, Paulo; Soker, Shay; Atala, Anthony; Keller, Daniel; Noiva, Rute; Carvalho, Sandra; Peleteiro, Conceição; Cabral, Joaquim M S; Eberli, Daniel; da Silva, Cláudia L; Baptista, Pedro M

doi:10.5167/uzh-135027

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Simões, I. N., Vale, P., Soker, S., Atala, A., Keller, D., Noiva, R., Carvalho, S., Peleteiro, C., Cabral, J. M. S., Eberli, D., da Silva, C. L., & Baptista, P. M. (2017). Acellular urethra bioscaffold: decellularization of whole urethras for tissue engineering applications. Scientific Reports, 7, 41934. https://doi.org/10.1038/srep41934

Abstract

Patients with stress urinary incontinence mainly suffer from malfunction of the urethra closure mechanism. We established the decellularization of porcine urethras to produce acellular urethra bioscaffolds for future tissue engineering applications, using bioscaffolds or bioscaffold-derived soluble products. Cellular removal was evaluated by H&E, DAPI and DNA quantification. The presence of specific ECM proteins was assessed through immunofluorescence staining and colorimetric assay kits. Human skeletal muscle myoblasts, muscle progen