Publication: Azidohomoalanine: A Minimally Invasive, Versatile, and Sensitive Infrared Label in Proteins To Study Ligand Binding
Date
Date
Date
2018
Journal Article
Published version
Abstract
Abstract
Abstract
The noncanonical amino acid azidohomoalanine (Aha) is known to be an environment-sensitive infrared probe for the site-specific investigation of protein structure and dynamics. Here, the capability of that label is explored to detect protein ligand interactions by incorporating it in the vicinity of the binding groove of a PDZ2 domain. Circular dichroism and isothermal titration calorimetry measurements reveal that the perturbation of the protein system by mutation is negligible, with minimal influence on protein stability and binding
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157 since deposited on 2019-02-05
Acq. date: 2025-11-12
Views
159 since deposited on 2019-02-05
Acq. date: 2025-11-12
Additional indexing
Creators (Authors)
- Zanobini, Claudio
- Bozovic, Olga
- Jankovic, Brankica
- Koziol, Klemens L
- Johnson, Philip J M
- Gulzar, Adnan
- Wolf, Steffen
- Stock, Gerhard
Volume
Volume
Volume
122
Number
Number
Number
44
Page range/Item number
Page range/Item number
Page range/Item number
10118
Page end
Page end
Page end
10125
Item Type
Item Type
Item Type
Journal Article
In collections
Dewey Decimal Classifikation
Dewey Decimal Classifikation
Dewey Decimal Classifikation
Keywords
Physical and Theoretical Chemistry, Materials Chemistry, Surfaces, Coatings and Films
Language
Language
Language
English
Publication date
Publication date
Publication date
2018-10-21
Date available
Date available
Date available
2019-02-05
ISSN or e-ISSN
ISSN or e-ISSN
ISSN or e-ISSN
1520-5207
Additional Information
Additional Information
Additional Information
This document is the Accepted Manuscript version of a Published Work that appeared in final form inJournal of Physical Chemistry B, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.jpcb.8b08368.
OA Status
OA Status
OA Status
Green
Publisher DOI
Metrics
Downloads
157 since deposited on 2019-02-05
Acq. date: 2025-11-12
Views
159 since deposited on 2019-02-05
Acq. date: 2025-11-12
Green Open Access
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Files
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Files
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