Publication: Fast and Sensitive Multiplex Real-Time Quantitative PCR to Detect Cutibacterium Periprosthetic Joint Infections
Fast and Sensitive Multiplex Real-Time Quantitative PCR to Detect Cutibacterium Periprosthetic Joint Infections
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Prinz, J., Schmid, B., Zbinden, R., Zingg, P. O., Uçkay, I., Achermann, Y., & Bosshard, P. P. (2022). Fast and Sensitive Multiplex Real-Time Quantitative PCR to Detect Cutibacterium Periprosthetic Joint Infections. Journal of Molecular Diagnostics, 24(6), 666–673. https://doi.org/10.1016/j.jmoldx.2022.03.003
Abstract
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Abstract
Diagnosis of Cutibacterium periprosthetic joint infections (PJIs) is challenging due to a long cultivation time of up to 14 days. Faster culture-independent diagnosis would improve patient care with early and accurate treatment. Specific primers and probes were designed for Cutibacterium acnes, Cutibacterium avidum, and Cutibacterium granulosum and evaluated in a multiplex TaqMan real-time quantitative PCR (qPCR) format on 57 skin swabs and 20 culture-negative cerebrospinal fluid samples. The multiplex qPCR was tested in a PJI cohort
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- Prinz, Julia
- Schmid, Bettina
- Zbinden, Reinhard
- Zingg, Patrick O
- Uçkay, Ilker
- Bosshard, Philipp P
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Prinz, J., Schmid, B., Zbinden, R., Zingg, P. O., Uçkay, I., Achermann, Y., & Bosshard, P. P. (2022). Fast and Sensitive Multiplex Real-Time Quantitative PCR to Detect Cutibacterium Periprosthetic Joint Infections. Journal of Molecular Diagnostics, 24(6), 666–673. https://doi.org/10.1016/j.jmoldx.2022.03.003
